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Life Sciences
Quantum Dots - A Versatile Research Tool in Life Science
EviTag Quantum Dot Labels and EviFluor Quantum Dot Conjugates are ideally suited for life science research applications and can be used to displace many of the applications currently accomplished via organic dyes and fluorophores.
Evident Technologies has the highest quality lipid coated quantum dots EviTags available for life science research applications. We also offer quantum dots conjugated to secondary antibodies and proteins EviFluors.
At Evident, we've developed protocols for the most common life science research applications for use with our quantum dots, including:
Applications and Protocols
EviFluors Quantum Dot Conjugates offer major advantages over traditional chemiluminescent and radioactive techniques used in Western Blot analysis. In addition to offering the ability to color multiplex, EviFluors also offers a significant cost savings compared to traditional chemiluminescence. Our In-Gel Western Blot Analysis protocol will significantly decrease the time you spend running Western Blot analysis.
EviFluor Quantum Dot Conjugates are ideally suited for flow cytometry applications. The number of emission colors made possible by quantum dots, as well as the possibility of eliminating multiple lasers sources of conventional flow cytometers and replacing with simplified LEDs and optics, greatly increase throughput and will reduce the costs of next generation flow cytometer systems.
EviTags Quantum Dot Labels and EviFluor quantum dot conjugates have been successfully introduced into live, fixed and permeabilized cells. They have been used to preferentially stain the cytoskeleton, centromeres and kinetocores, and other organelles as well as track the evolution of growth of frog and nematode embryos. Due to the unprecedented photostability and color multiplexing properties, continuous observations using fluorescence and confocal microscopes of multiple cellular components and cellular metabolism/transport can be made over a period of hours to days.
EviTag Quantum Dots Labels are excellent for ceullular uptake and cell tracking applications. Their high resistance to photobleaching allows them to remain bright for much longer than traditional organic fluorophores.
EviTags quantum dots are very efficient FRET donors with organic fluorophores, due to the large overlap between the quantum dot emission wavelength and the absorption spectra of the dyes. Because the emission characteristics of the EviTags can be continuously tuned, it is possible to create a FRET donor for any number of organic dyes that emit between approximately 510nm and 640nm.
EviTag Quantum Dot Labels have been introduced into large multicellular animals (mice) to preferentially stain the vascular and lymphatic systems, tumors etc. The photostability and ability to engineer quantum dot surfaces for injection into live animals opens the door to many in vivo studies and diagnostic applications. The quantum dots proved to be nontoxic and easily cleared by the kidneys and liver of the test organism.
EviTags Quantum Dot Labels have been successfully coupled to oligonucleotides and molecular beacons (oligonucleotide hairpins) and can serve as the basis for microarrays and other forms of DNA and RNA assays.
EviTags quantum dots conjugated to primary antibodies have been used to preferentially stain the surface of pathogenic E. Coli vs. nonpathogenic E. Coli and observed through microscopes and other instruments. Accurate, multiplexed assays to determine the presence of dangerous pathogens can be performed. Inexpensive detection platforms can be designed with far fewer laser and optical components because of the unique absorption and emission characteristics of quantum dots.
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