EviTag Specifications

EviTag Specifications
Emission (nm)	Material System	Size(nm)	FWHM(nm)	Recommended Excitation (nm)	1^st Exciton Peak (nm)	Molar Extinction Coefficient at First Exciton Peak (10⁵)
490	CdSe/ZnS	25	<40	<400	470	0.40
520	CdSe/ZnS		<35		505	0.56
540	CdSe/ZnS		<35		525	0.72
560	CdSe/ZnS		<35		545	0.98
580	CdSe/ZnS		<35		560	1.3
600	CdSe/ZnS		<35		585	2.3
620	CdSe/ZnS		<35		600	3.4
650	InGaP/ZnS		<40		-	6.7 5.3
680	InGaP/ZnS		<40		-	6.7 5.3

CdSe/ZnS EviTags, 490nm -620nm

CdSe/ZnS EviTags (Amine, Carboxyl or Non-Functional)
Description: Quantum dots with PEG lipid surface coating
Recommended Excitation [nm] <400
Emission [nm] 520 +/-10
Composition: CdSe/ZnS quantum dots, approx. hydrodynamic diameter 25nm
Solvent: DI Water
Storage: Sealed container, keep in dark, 4ºC, do not freeze
Shelf Life: 6 Months unopened

InGaP/ZnS EviTags, 650nm-680nm

InGaP/ZnS (Amine, Carboxyl or Non-Functional)
Description: Quantum dots with PEG lipid surface coating
Recommended Excitation [nm] <543
Emission [nm] 680 +/-15
Composition: InGaP/ZnS quantum dots, approx. hydrodynamic diameter 25nm
Solvent: DI Water
Storage: Sealed container, keep in dark, 4ºC, do not freeze
Shelf Life: 6 Months unopened Environmental Conditions

Environmental Conditions

Environmental Conditions		CdSe/ZnS EviTags	InGaP EviTags
pH Stability		5-11	5-11
Temperature (°C)		4-37	4-37
Photostability Under Imaging Coniditions (min.)¹		>75	>75
Chemical Environments		% Fluorescence Intensity after 1 Hour²
Buffers & Salts	PBS	= 98%	= 92%
	0.1XTE³	= 99%	= 88%
	0.1XTAE⁴	= 97%	= 95%
	0.1XSSC⁵	= 99%	= 94%
	1XTNT⁶	= 95%	= 97%
	0.1M Borate pH 8.4	= 96%	= 93%
	0.3M NaCl	= 87%	= 88%
Detergents	0.1% SDS	= 99%	= 99%
	0.1% Triton X-100	= 99%	= 96%
	1% NP-40	= 95%	= 83%
	0.1% Tween 20	= 99%	= 87%
Solvents	Methanol	= 80%	= 39%
Ethanol	= 62%	= 53%
DMSO⁷	= 95%	= 99%
DMF⁸	= 71%	= 65%
Others	40% Sucrose	= 96%	= 99%
	50% Glycerol	= 99%	= 99%
	Buffered formalin	= 94%	= 99%

Nikon Eclipse TS 100 fluorescence microscope with UV2A DM400 filter and 100W mercury lamp.
Percent of fluorescence intensity remaining after 1hour incubation at room temperature.
TE= 10mM Tris and 1mM EDTA at pH 8.0.
TAE= 40mM Tris acetate and 1mM EDTA at pH 8.3.
20X SSC=0.3M Sodium citrate, pH ~7.0, containing 3M NaCl.
TNT=10mM Tris-HCl, 150mM NaCl and 0.05% Tween 20.
DMSO=Methyl Sulfoxide
DMF=N,N-Dimethylformamide

Order EviTag Quantum Dot Labels Below

Product Availability
Surface	Wavelength	Part Number	Conc.	System	Price
Amine (AM2) Carboxyl (CB2) Non-Functionalized (NF2)	490nm	E2-C11-XXX-0490	12uM	CdSe/ZnS	500μL - $399
	520nm	E2-C11-XXX-0520
	540nm	E2-C11-XXX-0540
	560nm	E2-C11-XXX-0560
	580nm	E2-C11-XXX-0580
	600nm	E2-C11-XXX-0600
	620nm	E2-C11-XXX-0620
	650nm	E2-I61-XXX-0650	8uM	InGaP/ZnS	500μL - $449
	680nm	E2-I61-XXX-0680	8uM	InGaP/ZnS	500μL - $449
Specifications

EviTag Specifications