Filter Recommendations

Use of Narrow vs. Wide Excitation Filters with Quantum Dots

Wide bandpass excitation filters are used when the user needs to direct greater optical power onto the sample. This is particularly useful when observing low concentrations of target fluorophores. In situations where the concentration of fluorophores is large enough to swamp out other fainter signals or overwhelm the detector, a narrower excitation filter is desirable.

Conversely, narrow excitation filters are used in color multiplexing applications where a large number of fluorophores, each emitting at different wavelengths, are being observed simultaneously. In these situations, the excitation filter dedicated to one fluorophore cannot overlap the adjacent emission band of another fluorophore without giving false signal. In applications where only a single fluorophore is being observed a wider emission filter can be used to increase the amount of light reaching the detector resulting in increased signal.

Recommended & Alternative Filter Sets

Filter Type	Recommended Chroma Filter	Acceptable Alternative
Adirondack Green - 520nm Filter Sets
Excitation	p/n 32203 - 20nm Emission or p/n 32204 - 40nm Emission	DAPI Excitation
Dichroic		DAPI Dichroic
Emission		FITC Emission
Fort Orange - 600nm Filter Sets
Excitation	p/n 32211 - 20nm Emission or p/n 32212 - 40nm Emission	DAPI Excitation
Dichroic		DAPI Dichroic
Emission		CY3 Emission
Maple Red-Orange 620nm Filter Sets
Excitation	p/n 32213 - 20nm Emission or p/n 32214 - 40nm Emission	FITC Excitation
Dichroic		FITC Dichroic
Emission		Texas Red Emission
Jonamac Red - 680nm Filter Sets
Excitation	p/n 32217 - 20nm Emission or p/n32218 - 40nm Emission	FITC Excitation
Dichroic		CY5 Dichroic
Emission		CY5 Dichroic

Filters for EviTags Available From Chroma

To optimize visualization of EviTags, Evident recommends custom filter sets provided by Chroma Technology Corp. Chroma offers a set of filters that have been tuned to obtain the best results when using EviTags. To order filter sets please contact Chroma Technology Corp. at 1-800-8-CHROMA for more information or visit www.chroma.com/products to order online.

Narrow Excitation Filter Sets

Wavelength	Excitation Filter Wavelength (nm)	Bandwith (nm)	Dichroic Filter Wavelength (nm)	Type of Dichroic Filter	Emission Filter Wavelength (nm)	Bandwith (nm)
490nm	420	40	470	Long Pass, Extended Reflection	490	20
						40
520nm	420	40	470	Long Pass, Extended Reflection	520	20
						40
540nm	405	90	470	Long Pass, Extended Reflection	540	20
						40
	420	40				20
						40
560nm	405	90	470	Long Pass, Extended Reflection	560	20
						40
	420	40				20
						40
580nm	405	90	470	Long Pass, Extended Reflection	580	20
						40
	420	40				20
						40
600nm	405	90	470	Long Pass, Extended Reflection	600	20
						40
	420	40				20
						40
620nm	405	90	470	Long Pass, Extended Reflection	620	20
						40
	420	40				20
						40
680nm	405	90	470	Long Pass, Extended Reflection	680	20
						40
	420	40				20
						40

Wide Excitation Filter Sets